Effect of Parasite Diversity and Age on Antibody Responses

Effect of Parasite Diversity and Age on Antibody ResponsesThis scan was aimed at investigating the effect of parasite regeneration and while on the levels of antibody responses to P. falciparum in individuals living in an area of temperal malaria transmission system. Two blood st come on antigens (MSP1(19), AMA1) and two liver stage antigens (CSP and celTOS), making a total of four antigens were selected to be used for the vignette. These antigens were tested in archived plasma samples with ages ranging from 1 year to 70 years. The samples were collected close to the end of the rainy season in the months of November and celestial latitude ( prankish season), and April the following year at the end of the dry season (dry season).Of the samples collected in the awry(p) season, 34.1% had parasites whiles those collected in the dry season was 15.5% (table 1). Appawu (2004) report the seasonality of malaria transmission in a neighbouring district (Kasena Nankana District), being hi gh in the potent season and low in the dry season. The high transmission in the wet season could be due to favourable breeding grounds for mosquitoes as a result of several collections of water when it rains and in turn increase the hail of vectors and hence the high event of parasites individuals are exposed to (Ahmed, 1989). This selective information is in agreement with an earlier considering in northern gold coast by Apawu and his class where they found high parasite heading, and high multiplicity of infection (MOI) in the wet season compared to low carriage and MOI in the dry season. Parasite carriage here means the proportion of participants with P. falciparum at enrolment. on that point was however no statistically significant difference in the parasite densities when compared amidst the seasons (p=0529) table 1. There was no difference in the overall ages between the wet and the dry season. (p=0.937). This was expected as most of the samples collected were from the same individuals in the two seasons.To look age related pattern of P. falciparum infection, participants were categorized into three (3) age assorts under 5 years, between 5 and 15 years, and over 15 years. The data shows the 5-15 year olds having higher(prenominal) infected proportion in the two seasons (table 2). Parasitaemia was as well determined using a more sensitive method, the polymerase chain reaction (PCR). The PCR data confirmed the microscopy data be practise the 6-15 year group besides had higher proportion of individuals carrying parasites at enrolment. This could be due to the fact that they are the more exposed group with incompletely developed immune systems. In contrast, under-fives who are much younger are likely to receive better care from parents thus preventing them from being infected with the parasites. Bed net usage could be one way of protecting the children. Also because of their younger ages they may not be allowed roam around freely. Adults on the ot her hand despite having possible similar exposure as 5-15 year olds, seem to have less parasites because of their developed anti-malarial immunity. This partial immunity is gained with age and repeated exposure thus making the younger individuals more susceptible to infection (Pratt-Riccio et al., 2005 Dodoo et al., 2008). Thus the over 15 year group who are older, had the least proportion of infection (table 2).The study also sought to determine the relationship between IgG levels and age. Figures fig 7 and 8 shows the correlational statistics plots between age and IgG levels in the wet and dry season respectively. There was a significant positive correlation between and IgG levels raised against AMA1-FVO, MSP1, CSP and CelTOS in the wet season. And for the dry season, the significant correlation was between age and IgG levels to AMA1-FVO, MSP1, CSP, and Celtos. The correlation coefficient (r2) and the p values are indicated on each plot. There was however no correlation between a ge and AMA1-3D7 (r2 0.0142, p 0.0699) for the wet season, and AMA1-FVO (r2=0.0114, p= 0.0594) for the dry season. The significant correlations were however very weak. This indicates that though IgG levels increased with age, the association between the antibody levels and age was not so strong and that the influence of age on the amount of antibodies produce against most of the antigens in the existence studied was not that much. until now the age dependent increase in IgG response to the antigens tested may be due to the mature immune system in adults, and could also be due to cumulative exposure to infection over time (Nebie 2008). It is however not known what might be the reason for the lack of correlation with age to AMA1-3D7, and AMA1-FVO, in the wet and dry season respectively.IgG responses to the two AMA1 alleles (3D7, and FVO) was plotted against each other to determine which is more predominant in the study population. There was a strong correlation between the antibody l evels of AMA1 alleles of 3D7 and FVO (r2=0.8382, p) implying that both strains are circulating in the study site during the wet season. A similar arc was observed in the dry season but with a lower correlation coefficient compared to the wet season (r2= 0.5302, p).Antibody titres against recombinant antigens have often been linked to protection from clinical disease (Polley 2004, Nebie 2008, Dodoo 2008, Dodoo 1999, Cavanagh 2004). Where high anti-malaria antibodies is interpreted to mean protection from clinical disease. The important role of antibodies was demonstrated by the passive transfer of purified IgG from immune donors to individuals with P. falciparum infection, which trim parasitaemia (Cohen 1961, sabchareon 1991). Apical membrane antigen-1 AMA1 and MSP119 have been associated with reduced risk of clinical malaria (Branch 1998, Osier et al 2008). Anti-CSP antibodies have also been found to be partially protective where in Kenya high anti-CSP, anti-LSA, and anti-TRAP ( p re-erythrocytic ) antibodies were demonstrated to be associated with relative protection from reinfection (John CC 2005, 2008). The most advance malaria vaccine, RTSS which is a subunit of CSP, has been found to protect to about 35-55% in children 5-17 months for about 8 months (Alonso 2004,) . Also anti-CelTOS antibodies have been shown to inhibit invasion of herpatocytes by sporozoites in mice (Bergmann-Leitner 2010). In this study, antibody responses to the antigens, AMA1-3D7, AMA1-FVO, MSP119, CSP, and CelTOS were determined using indirect ELISA. The optical densities (OD) of the antigens were converted to positive units (AU) where the highest OD for each antigen was awarded an arbitrary unit of 4, to allow for comparison between antigens the antigens tested. Antibodies to AMA1 in individuals living in malaria indigenous regions have been reported to be high, (Thomas,1994, chelimo, 2005), and this study thus reports high antibodies to AMA1-3D7, and AMA1-FVO compared to MSP1(19 ), CSP, with anti-CelTOS antibodies being the least. (Fig 3) in both wet and dry seasons. The data also shows total IgG responses in the wet season was higher than the in the dry season for all the antigens (p) except MSP119 where no statistical differences was found between the two seasons (p=0.85) (Figure 3). The seasonal changes in antibody response could be as a result of higher number of vector (mosquitoes) in the community in the wet season which could in turn increase the exposure of the population to parasites and also introduce new parasite clones/strains in the population and hence the high anti-malaria antibodies found in the wet season. In contrast, the dry season cause a reduction in the number of breeding sites for the vectors reducing their population resulting in minimal exposure and hence the low antibody levels.In malaria endemic regions, the number of different clones of Plasmodium falciparum parasites infecting a person could be a transmission indicator, an indic ator of the hosts immune status, and a useful parameter in evaluating malaria control interventions (Babiker 1999, Arnot 1998, mayengue 2009). It has also been reported that parasite diversity in high malaria transmission areas are high and that individuals could carry multiple genotypes (clones) but the opposite pertains in low endemic areas with most infections being monoclonal (Peyerl-Hoffmann 2001, Bakiker 1997, Haddad 1999). Genotyping was done using block 3 region of the MSP 2 gene (Smythe et al. 1990) because of its high polymorphic nature (Felger et al.,1994 1999 Robert et al., 1996). Also MSP2 was selected to be used for this study because of the high allelic diversity observed in Ghana and other countries bordering Ghana with as many as 154 alleles in Ghana and about 50 genotypes in Cte dIvoire. ( Silue 2006, falk 2006). The results from the two seasons using the 3D7 allelic family primers showed high mean multiplicity of infection in the wet season compared to the dry sea son (1.76, and 1.46, p=0.001) table 3. In both seasons, the number of infections per person ranged from 0 to 4. A similar degree of multiple infections has been reported in other African settings (Ntoumi et al., 1995,Beck 1997, Engelbrecht 1999). The samples used for this study was collected in asymptomatic individuals and the high multiplicity of infection found in the wet season could probably be due to high rate of exposure as a result of favourable breeding conditions compared to the dry season. This study did not find any significant correlation between MOI and age in both seasons (p=0.5768 for wet season, and p=0.4158 for the dry season). The samples were then grouped into two found on whether positive or negative using PCR to detect Plasmodium parasites. No differences were detected in the IgG levels between the antigens tested in both seasons when compared based on the PCR data. This result is similar to that found when microscopy was used except that anti-CelTOS IgG in the parasitaemic group was higher than the non-parasitaemic group when microscopy was used. The disparity in the CelTOS report could be due to the lower sample size analysed using PCR compared to the microscopy.Apical membrane antigen AMA1 has been found to be a promising blood stage vaccine scene antigen but this potential has been dampen due to extensive polymorphism (Remaque 2008).